非洲猪瘟ASFV P72-Strep

PRODUCT SPECIFICATIONS

Product Name:    ASFV major capsid protein P72 (C-Strep Tag)

P72Description:        A DNA sequence encoding the major capsid protein P72 of African swine fever virus (ASFV) was expressed with Strep tag at the C-terminus.

Catalog #:            Lot #: 2020110301

Source:                 SF9 Insect cell

Format:             Purified, Liquid

Concentration:     2mg/ml

Purity:                   ≥95% (SDS-PAGE)

Product Buffer:   PBS, pH7.4

Preservative:      None                

Applications:      Suitable for use in immunoassays. Each laboratory should determine an optimum working titer for use in its particular applications.

Storage:              Store at -20°C to -80°C. Avoid multiple freeze/thaw cycles.

Expiry Date: 20211102

 

Indirect ELISA Sensitivity (1ug/ml coating Ag):

 

P72

P54

P30

1:100

2.92

2.72

2.82

1:1000

2.77

2.05

2.13

1:2000

2.49

1.82

1.89

1:4000

2.25

1.18

1.25

1:8000

1.84

0.72

0.82

1:16000

1.25

0.38

0.49

Negative Serum

0.079

0.065

0.071

 

Sample Test:

Test Method

P72

P54

P30

Positive rate

Negative rate

Positive rate

Negative rate

Positive rate

Negative rate

Indirect ELISA

 

28%

(34/121)

 

72%

(87/121)

 

20.7%

(25/121)

 

 

79.3%

(96/121)

 

 

25.6%

31/121

 

74.4%

90/121

 

 

Note: Centrifuge before opening to ensure complete recovery of vial contents

FOR RESEARCH OR FURTHER MANUFACTURING USE ONLY